Observaciones de crseaquist

Gathered dry leaves on 2024-02-23 and stored in water.

Here are some videos:
https://youtu.be/8DMTa0ZruLc
https://youtu.be/izC2XI3pRPY
https://youtu.be/1Q_9wdLj5pc

I think the videos seem to show it is an eruptive morphotype.

A large mature sporangium formed from a large plasmodium in a petri dish. The second image shows individual spores after they were moved to a slide. Last image possibly shows germination, the beginning of the emergence of a haploid amoeba.

Gathered dry leaves on 2024-02-23 and stored in water.
Two daughters emerging from a division cyst:

1. Initial observation of cyst with another individual
2. 2 minutes later
3. 18 minutes later
4. 77 minutes later
5. 84 minutes later
6. 140 minutes later
7. 153 minutes later
8. 154 minutes later 1st daughter emerges
9. 155 minutes later
10. 162 minutes later
11. 163 minutes later 2nd daughter emerges
12. 2nd daughter swims away

Video: https://youtu.be/vntikKDmSDE

Water sample taken from the edge of a freshwater playa on 2023-12-30 using a turkey baster.
Gliding and swimming forms:
Length of cell: 40 µm
Width of cell: 12 µm
Length of flagellum: 120 µm

Here is a video showing the organism changing from stationary to gliding to swimming forms:
https://youtu.be/6rgXXILDj8I

See observation https://www.inaturalist.org/observations/198088142 which shows a stationary form where movement is exceedingly slow by “eruptive” lobopodia.

Recently this organism has been sequenced:

“Phylogenetic position of the pelobiont Mastigamoeba aspera and revision of the family Mastigamoebidae (Archamoebae, Pelobiontida)” by Ludmila V. Chistyakova, et. al. (see https://www.sciencedirect.com/science/article/abs/pii/S0932473923000743?via%3Dihub)

This work has resulted in changes to the taxonomy and the introduction of new subfamilies, etc.

Water sample taken from the edge of a freshwater playa on 2023-12-30 using a turkey baster.

Flagellated amoeba crawling very slowly. (Mastigamoba, Type M. aspera Schulze 1875)
https://youtu.be/aYzgIXkhUx0

“Phylogenetic position of the pelobiont Mastigamoeba aspera and revision of the family Mastigamoebidae (Archamoebae, Pelobiontida)” by Ludmila V. Chistyakova, et. al. (see https://www.sciencedirect.com/science/article/abs/pii/S0932473923000743?via%3Dihub)

Water sample taken from the edge of a freshwater playa on 2023-12-07 using a turkey baster.

The images show the flattened ovoid shape of the test with a lens like cross section. No rod like spines are visible. There do appear to be xenosomes. The third image seems to show a CV and nucleus. It seems to be between 30 and 40 microns long.

Videos:
https://youtu.be/rNY33wLxCXo
https://youtu.be/BZW5JillJPo

Water sample taken from the edge of a freshwater playa on 2023-12-07 using a turkey baster.

From 1st image to last was about 25 minutes. Unfortunately I lost them when I tried to rehydrate the slide, so I never saw them actually separate.

Water sample taken from the edge of a freshwater playa on 2023-12-07 using a turkey baster.

Appears to me to be Fan-shaped morphotype.

Here is a video of a similar individual in the same sample: https://youtu.be/gNJhlXhcj-M

Water sample taken from the edge of a stagnate freshwater playa on 2023-11-06 using a turkey baster.

i. Diameter: 133 µm
ii. Aperture: 59 µm
iii. Height: ? µm
iv. A/D: 0.44

Does image 4 really show so many nuclei?

What is image 5 showing?